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Kidney Research pp 237–249Cite as

SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 466))

Abstract

Protein profiling has numerous applications in renal research including the detection of protein biomarkers with aberrant expression levels during disease development. Such information is essential for early diagnosis and will aid the improvement of patient management and minimise the progression of disease. Further to this, data generated from these studies will assist the elucidation of the precise mechanisms of disease development and can lead to the discovery of potential drug targets. Surface enhanced laser desorption/ionisation time of flight mass spectrometry (SELDI-TOF MS), is emerging as a popular profiling tool for such studies. It incorporates the methods of solid-phase chromatography and TOF-MS in a single platform. This chapter provides a guide for establishing kidney profiling experiments using SELDI-TOF MS and will cover the following topics: 1) preparation of tissue extracts; 2) array processing, including optimisation of conditions for biomarker discovery; and 3) data acquisition/analysis.

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Acknowledgments

This work was supported in part by a National Health & Medical Research Council (NHMRC) R.D.Wright/National Heart Foundation of Australia (NHFA) Career Development Fellowship to C.S.S and an Ian Potter Foundation grant to the Howard Florey Institute.

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Authors and Affiliations

  1. Howard Florey Institute, The University of Melbourne, Victoria, Australia and Bio-Rad Laboratories, Hercules, CA, USA

    Eleni Giannakis

  2. Howard Florey Institute, The University of Melbourne, Melbourne, Victoria, Australia

    Chrishan S. Samuel

  3. Department of Physiology, Monash University, Clayton, Melbourne, Victoria, Australia

    Wee-Ming Boon

  4. Howard Florey Institute, The University of Melbourne, Victoria, Australia

    Mary Macris

  5. Department of Nephrology, The Royal Melbourne Hospital and Department of Medicine, University of Melbourne, Melbourne, Victoria, Australia

    Tim D. Hewitson

  6. Howard Florey Institute, The University of Melbourne, Melbourne, Victoria, Australia

    John D. Wade

Authors
  1. Eleni Giannakis
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  2. Chrishan S. Samuel
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  3. Wee-Ming Boon
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  4. Mary Macris
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  5. Tim D. Hewitson
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  6. John D. Wade
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Giannakis, E., Samuel, C.S., Boon, WM., Macris, M., Hewitson, T. ., Wade, J. . (2009). SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue. In: Becker, G., Hewitson, T. (eds) Kidney Research. Methods in Molecular Biology, vol 466. Humana Press. https://doi.org/10.1007/978-1-59745-352-3_17

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    • DOI: https://doi.org/10.1007/978-1-59745-352-3_17

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-58829-945-1

    • Online ISBN: 978-1-59745-352-3

    • eBook Packages: Springer Protocols

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