Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions

  • Shu-ichi Matsuzawa
  • John C. Reed
Part of the Methods in Molecularbiology™ book series (MIMB, volume 383)


An important step in the analysis of protein function is identification of the interaction partners of each protein. The two-hybrid system has been widely used to identify and explore protein-protein interactions. By using various two-hybrid systems, numerous protein interactions that regulate apoptosis signaling have been discovered that reveal unexpected functions of cancer-relevant proteins. Methods for performing two-hybrid experiments using either yeast or mammalian cells will be described in this chapter.

Key Words

Mammalian two-hybrid system protein interaction yeast two-hybrid system prey bait reporter 


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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Shu-ichi Matsuzawa
    • 1
  • John C. Reed
    • 1
  1. 1.Burnham Institute for Medical ResearchLa Jolla

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