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Reciprocal Subtraction Differential RNA Display (RSDD)

An Efficient Technology for Cloning Differentially Expressed Genes
  • Devanand Sarkar
  • Dong-chul Kang
  • Paul B. Fisher
Part of the Methods in Molecularbiology™ book series (MIMB, volume 383)

Abstract

Identification of differentially expressed genes is an essential step in comprehending the molecular basis of complex physiological and pathological processes. Subtraction hybridization and differential RNA display (DDRT-PCR) are two methods that are widely and successfully employed to clone differentially expressed genes. Unfortunately, both methods have inherent problems and limitations requiring improvements in the technique. A combination of these two methods termed reciprocal subtraction differential RNA display is described here that considerably reduces the complexity of DDRT-PCR and facilitates the rapid and efficient identification and cloning of both abundant and rare differentially expressed genes.

Key Words

Cloning differential RNA display reciprocal subtraction differential RNA display RSDD subtraction hybridization tumor progression gene expression 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Devanand Sarkar
    • 1
  • Dong-chul Kang
    • 2
  • Paul B. Fisher
    • 1
    • 3
    • 4
    • 5
  1. 1.Department of UrologyColumbia University Medical Center, College of Physicians and SurgeonsNew York
  2. 2.Ilsong Institute of Life ScienceHallym UniversityAnyang, Kyeonggi-doRepublic of Korea
  3. 3.Herbert Irving Comprehensive Cancer CenterCollege of Physicians and Surgeons, Columbia University Medical CenterNew York
  4. 4.Department of PathologyCollege of Physicians and Surgeons, Columbia University Medical CenterNew York
  5. 5.Department of NeurosurgeryCollege of Physicians and Surgeons, Columbia University Medical CenterNew York

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