Abstract
A conventional flow cytometry procedure for creating single cell suspensions is provided. Methods of tissue disaggregation to create single-cell suspensions from formalin fixed tissue are also given. Chemical, mechanical, and enzymatic multistep methods for separating single cells from tissue are discussed. These procedures are very tissue dependent.
The opinions or assertions herein represent the personal view of the author and are not to be construed as official or as representing the views of the Department of the Army or the Department of Defense
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Cunningham, R.E. (2010). Tissue Disaggregation. In: Oliver, C., Jamur, M. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology, vol 588. Humana Press. https://doi.org/10.1007/978-1-59745-324-0_32
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DOI: https://doi.org/10.1007/978-1-59745-324-0_32
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Publisher Name: Humana Press
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Online ISBN: 978-1-59745-324-0
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