Abstract
About 40 yr ago, two groups of investigators identified a new class of immunoglobulins, IgE. By exchanging their results and reagents, they proved that the immunoglobulin responsible for immediate hypersensitivity was IgE. From that day forward the science of allergy was greatly advanced. Within a few years of the IgE discovery, an assay for IgE was developed. This test was named the radio allergosorbent test. The specific IgE testing methodology has matured in the last four decades. Different means of detecting IgE bound to allergen is the subject of this review. We have included methods for measuring specific IgE using the ImmunoCAP 1000 instrument. The methodology for measuring basophile histamine release is also detailed in this chapter.
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References
Ishazaka, K. and Ishizaka, T. (1967) Physiochemical properties of reaginic antibody. I. Association of reaginic activity with an immunoglobulin other than gamma A or gamma G globulin. J. Allergy. 37, 169.
Bennich, H., Ishizaka, K., Ishizaka, T., and Johansson, S. G. O. (1969) A comparative antigenic study of gamma E globulin and myeloma IgND. J. Immunol. 102, 826–831.
Wide, L., Bennich, H., and Johansson, S. G. O. (1967) Diagnosis of allergy by an in vitro tests for allergen-specific IgE antibodies. Lancet 2, 1105–1107.
Paganelli, R., Ansoteugi, I. J., Sastre, J., et al. (1998) Specific IgE antibodies in the diagnosis of atopic disease. Clinical evaluation of a new in vitro test system, UniCAP, in six European allergy clinics. Allergy 53, 763–768.
Ownby, D. R. and McCullough, J. (1994) Testing for latex allergy. J. Clin. Immunoassay 16, 109–113.
Biagini, R. E., Krieg, E. F., Pinkerton, L. E., and Hamilton, R. G. (2001) Receiver operating characteristics analyses of Food and Drug Administration-cleared serological assays for natural rubber latex-specific immunoglobulin E antibody. Clin. Diagn. Lab. Immunol. 8, 1145–1149.
Hamilton, R. G. and Franklin Adkinson, N., Jr. (2004) In vitro assays for the diagnosis of IgE-mediated disorders. J. Allergy Clin. Immunol. 114, 213–225.
Crockard, A. D. and Ennis, M. (2001) Basophil histamine release tests in the diagnosis of allergy and asthma. Clin. Exp. Allergy 31, 345–350.
Nolte, H. (1993) The clinical utility of basophil histamine release. Allergy Proc. 14, 251–254.
Maly, F. E., Marti-Wyss, S., Blumber, S., Cuhat-Stark, I., and Wuthrich, B. (1997) Mononuclear blood cell sulpholeukotriene generation in the presence of interleukin 3 and whole blood histamine release in honeybee and yellow jacket venom allergy. J. Invest. Allergy Clin. Immunol. 7, 217–224.
Siraganian, R. P. (1974) An automated continuous-flow system for the extraction and fluorometric analysis of histamine. Anal. Biochem. 57, 383–394.
Boumiza, R., Monneret, G., Forissier, M. F., et al. (2003) Marked improvement of the basophil activation test by detecting CD203c instead of CD63. Clin. Exp. Allergy 33, 259–265.
Ebo, D. G., Hagendorens, M. M., Bridts, C. H., Schuerwegh, A. J., De Clerk, L. S., and Stevens, W. J. (2004) In vitro allergy diagnosis: should we follow the flow? Clin. Exp. Allergy. 34, 332–339.
Hiller, R., Laffer, S., Harwanegg, C., et al. (2002) Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment. FASEB J. 16, 414–416.
Jahn-Schmid, B., Harwanegg, C., Hiller, R., et al. (2003) Allergen microarray: comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen-specific serum immunoglobulin E. Clin. Exp. Allergy 33, 1443–1449.
Sampson, H. A. and Ho, D. G. (1997) Relationship between food-specific IgE concentrations and the risk of positive food challenges in children and adolescents. J. Allergy Clin. Immunol. 100, 444–451.
Lichtenstein, L., Norman, P., and Winkenwereder, W. (1968) Clinical and in vitro studies on the role of immunotherapy in ragweed hay fever. Am. J. Med. 44, 514–524.
Adkinson, N. F., Jr. (1976) Measurement of Total Serum Immunoglobulin E and Allergen-Specific Immunoglobulin E Antibody. In: Manual of clinical laboratory Immunology, (Rose, N. R. and Friedman, H., eds.), American Society for Microbiology, Washington, DC, p. 590.
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© 2007 Humana Press Inc., Totowa, NJ
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Glovsky, M.M. (2007). Measuring Allergen-Specific IgE. In: Albitar, M. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 378. Humana Press. https://doi.org/10.1007/978-1-59745-323-3_15
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DOI: https://doi.org/10.1007/978-1-59745-323-3_15
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