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Fluorescence In Situ Hybridization on Single Cells

Sex Determination and Chromosome Rearrangements

  • Protocol
Single Cell Diagnostics

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 132))

Abstract

Fluorescence in situ hybridization (FISH) is the technique of choice for preimplantation genetic diagnosis (PGD) selection of female embryos in families with X-linked disease, for which there is no mutation-specific test. FISH with target-specific DNA probes is also the primary technique used for PGD detection of chromosome imbalance associated with Robertsonian translocations, reciprocal translocations, inversions, and other chromosome rearrangements, because the DNA probes, labeled with different fluorochromes or haptens, detect the copy number of their target loci. The methods described outline strategies for PGD for sex determination and chromosome rearrangements. These methods are assessment of reproductive risks, the selection of suitable probes for interphase FISH, spreading techniques for blastomere nuclei, and in situ hybridization and signal scoring using directly labeled and indirectly labeled probes.

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Author information

Authors and Affiliations

  1. Research and Development, Guy’s & St Thomas’ NHS Foundation Trust Centre for PGD, London, UK

    Paul N. Scriven PhD & Caroline Mackie Ogilvie DPhil

  2. Cytogenetics Department, Guy’s Hospital, London, UK

    Paul N. Scriven PhD & Caroline Mackie Ogilvie DPhil

Authors
  1. Paul N. Scriven PhD
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  2. Caroline Mackie Ogilvie DPhil
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Editor information

Editors and Affiliations

  1. The London Bridge Fertility, Gynecology and Genetics Centre, London, UK

    Alan Thornhill PhD, HCLD

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© 2007 Humana Press Inc., Totowa, NJ

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Scriven, P.N., Ogilvie, C.M. (2007). Fluorescence In Situ Hybridization on Single Cells. In: Thornhill, A. (eds) Single Cell Diagnostics. Methods in Molecular Medicine™, vol 132. Humana Press. https://doi.org/10.1007/978-1-59745-298-4_3

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  • DOI: https://doi.org/10.1007/978-1-59745-298-4_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-578-1

  • Online ISBN: 978-1-59745-298-4

  • eBook Packages: Springer Protocols

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