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Plant Signal Transduction pp 35–59Cite as

Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 479))

Abstract

The disruption or modulation of signal transduction pathways does not always lead to drastic changes in plant growth and development. Therefore, many loss- or gain-of-function lines do not exhibit an obvious phenotype under normal greenhouse conditions. To be able to assign biological functions to these genes, the mutants need to be evaluated with a broad spectrum of assays to uncover conditional phenotypes.

Here we provide an overview on how to evaluate plants in their development and their response to abiotic factors such as light, hormones, and different stressors. The assessment ofthe behavior ofa plant under these conditions can be used to correlate a biological role with a genotype. This phenotypic analysis can be used for profiling of mutants.

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Author information

Authors and Affiliations

  1. Ludwig-Maximilians-Universität, Department für Biologie I Lehrstuhl für Botanik, München, Germany

    Cordelia Bolle

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  1. Cordelia Bolle
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Editor information

Editors and Affiliations

  1. Institut für Allgemeine Botanik und Pflanzenphysiologie, Lehrstuhl Pflanzenphysiologie, Friedrich-Schiller-Universität Jena, Jena, Germany

    Thomas Pfannschmidt

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Bolle, C. (2009). Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis . In: Pfannschmidt, T. (eds) Plant Signal Transduction. Methods in Molecular Biology, vol 479. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-289-2_3

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  • DOI: https://doi.org/10.1007/978-1-59745-289-2_3

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-943-7

  • Online ISBN: 978-1-59745-289-2

  • eBook Packages: Springer Protocols

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