Summary
Postprandial blood glucose homeostasis is regulated by an insulin-stimulated increase in glucose transport into muscle and fat tissues via glucose transporter isoform 4 (GLUT4). In the basal state, this constitutively recycling membrane protein predominantly resides intracellularly. In order to achieve the insulin-stimulated increase in glucose flux, GLUT4 increases its cell surface abundance at the expense of preformed intracellular depots. By confocal microscopy of cultured L6 muscle cells stably expressing myc-tagged GLUT4 (L6-GLUT4myc), we can visualize the two arms of GLUT4 traffic: exocytosis (movement to the cell surface) and endocytosis (internalization from the cell surface).
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Acknowledgments
The authors would like to thank Professor Y. Ebina for help in the development of the stable L6-GLUT4myc cell line and Dr. P. Bilan for the careful reading of this manuscript. This work was supported by a Canadian Institutes of Health Research (CIHR) grant (MT7307) to AK. C.N.A. has received doctoral studentship funding from an Ontario Graduate Scholarship (OGS) and a Doctoral Research Award from the Canadian Diabetes Association. V.K.R. has received doctoral studentship funding from CIHR, Banting and Best Diabetes Centre-Novo Nordisk and University of Toronto, an OGS, a SickKids Research Training Centre-University of Toronto MD/PhD Award, and a Dr. Joseph A. Connolly Award in Cell Biology.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Antonescu, C.N., Randhawa, V.K., Klip, A. (2008). Dissecting GLUT4 Traffic Components in L6 Myocytes by Fluorescence-Based, Single-Cell Assays. In: Vancura, A. (eds) Membrane Trafficking. Methods in Molecular Biology, vol 457. Humana Press. https://doi.org/10.1007/978-1-59745-261-8_27
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DOI: https://doi.org/10.1007/978-1-59745-261-8_27
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