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Immunocytochemistry on Suprachiasmatic Nucleus Slices

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Circadian Rhythms

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 362))

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Abstract

Immunocytochemistry (ICC) is a sensitive and powerful method that is used to localize and identify cells containing a particular antigen. This chapter is dedicated to ICC of suprachiasmatic nucleus (SCN) slices. After a brief introduction to the technique, the materials and methods sections describe two different methods to obtain SCN slices—the first one for fixed tissue, the second one for fresh frozen tissue—followed by the description of two methods of antibody detection: the indirect method and the avidinbiotin complex one. In addition, some remedies to the most common problems encountered while performing ICC and some alternative protocols are discussed.

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References

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Authors and Affiliations

  1. Department of Molecular and Integrative Neuroscience, Imperial College London, London, UK

    Marta Muñoz Llamosas

Authors
  1. Marta Muñoz Llamosas
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Editor information

Editors and Affiliations

  1. Department of Genetics, University of Leicester, Leicester, UK

    Ezio Rosato

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© 2007 Humana Press Inc.

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Llamosas, M.M. (2007). Immunocytochemistry on Suprachiasmatic Nucleus Slices. In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_43

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  • DOI: https://doi.org/10.1007/978-1-59745-257-1_43

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-417-3

  • Online ISBN: 978-1-59745-257-1

  • eBook Packages: Springer Protocols

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