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Immunohistochemistry in Drosophila

Sections and Whole Mounts
  • Charlotte Helfrich-Förster
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

This chapter describes immunohistochemistry on Drosophila heads (brains) with respect to antigens involved in the circadian system. Two different methods have been successfully performed in several labs: immunolabeling on whole-mount brains and on cryostat sections of entire heads. Both methods are addressed here. The primary antisera can be detected by enzyme-labeled or fluorescence-labeled secondary antibodies. The advantages of the different methods are discussed.

Key Words

Antigen antiserum brain circadian rhythms compound eyes cryostat sections Drosophila fluorescence period horseradish peroxidase immunohistochemistry peroxidase-antiperoxidase secondary antibody timeless whole mounts 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Charlotte Helfrich-Förster
    • 1
  1. 1.Institut für ZoologieUniversität RegensburgRegensburgGermany

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