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Reporter Assays

  • M. Fernanda Ceriani
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

Transcriptional feedback loops are at the core of the molecular clockworks. As single clock genes were cloned it was compelling to develop an assay that allowed simple and direct functional testing of putative activators or repressors of transcription. This chapter includes a general description and guidelines to carry out transcriptional assays in transiently transfected Schneider’s cells.

Key Words

Transcriptional assays firefly luciferase Renilla luciferase S2 cells 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • M. Fernanda Ceriani
    • 1
  1. 1.Department Behavioral GeneticsFundación Instituto LeloirBuenos AiresArgentina

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