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Western Blotting

  • Choogon Lee
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

Western blotting is one of the most commonly used biochemical techniques to detect a specific protein from a mixture of proteins such as tissue extracts. Antibodies to the specific antigen are used to detect the protein. The mixture of proteins is resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a membrane. A specific antigen immobilized on the membrane is detected and visualized by a primary antibody, a secondary antibody-peroxidase conjugate, and a chemiluminescent reagent.

Key Words

Western blotting SDS-PAGE primary antibody secondary antibody chemiluminescence 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Choogon Lee
    • 1
  1. 1.Department of Biomedical SciencesFlorida State University College of MedicineTallahassee

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