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Plant Protein Extraction

  • Helen E. Conlon
  • Michael G. Salter
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

A method is presented for the extraction of total protein from Arabidopsis thaliana tissue. The protocol was designed for the solubilization of a range of proteins and their efficient and quantitative recovery. It is especially compatible with the small quantities of available tissue often associated with this species and was originally intended for Western blot preparations. Samples extracted using this method can be quantitated directly using a commercially available kit.

Key Words

Arabidopsis thaliana total protein extraction efficient limited tissue Western blot 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Helen E. Conlon
    • 1
  • Michael G. Salter
    • 1
  1. 1.Department of BiologyUniversity of LeicesterLeicesterUK

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