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Protein Extraction From Drosophila Heads

  • Patrick Emery
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

In Drosophila, the concentration and phosphorylation levels of several important circadian proteins (e.g., PERIOD, TIMELESS) oscillate on a 24-h basis. A simple and rapid method for extracting proteins from fly heads is presented here. The extracts can immediately be loaded onto an sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel to assay the effects of mutations, genetic manipulations, or environmental conditions on the oscillations of circadian proteins by Western blotting. They can also be used for immunoprecipitation experiments.

Key Words

Protein extraction protein cycling phosphorylation immunoprecipitation 

References

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Patrick Emery
    • 1
  1. 1.Department of NeurobiologyUniversity of Massachusetts Medical SchoolWorcester

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