Abstract
The RNase protection assay is a standard approach to determine mRNA levels of a gene of interest in different tissues, developmental stages, or times of the day. Splicing or promoter variants can be studied with specific probes. It is widely used in chronobiology to study the temporal profile of expression of circadian genes and the effects of genetic manipulation on these oscillations. Methods to generate the riboprobes and to perform the RNase protection assay itself are described in this chapter.
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References
Hardin, P. E., Hall, J. C., and Rosbash, M. (1990) Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levels. Nature 343, 536–540.
Sehgal, A., Rothenfluh-Hilfiker, A., Hunter-Ensor, M., Chen, Y., Myers, M., and Young, M. W. (1995) Circadian oscillations and autoregulation of timeless RNA. Science 270, 808–810.
Balsalobre, A., Damiola, F., and Schibler, U. (1998) Immortalized rat fibroblasts contain a circadian clock. Cell 93, 929–937.
Emery, P., So, W. V., Kaneko, M., Hall, J. C., and Rosbash, M. (1998) CRY, a Drosophila clock and light-regulated cryptochrome, is a major contributor to circadian rhythm resetting and photosensitivity. Cell 95, 669–679.
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© 2007 Humana Press Inc.
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Emery, P. (2007). RNase Protection Assay. In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_24
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DOI: https://doi.org/10.1007/978-1-59745-257-1_24
Publisher Name: Humana Press
Print ISBN: 978-1-58829-417-3
Online ISBN: 978-1-59745-257-1
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