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Circadian Rhythms pp 343–348Cite as

RNase Protection Assay

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 362)

Abstract

The RNase protection assay is a standard approach to determine mRNA levels of a gene of interest in different tissues, developmental stages, or times of the day. Splicing or promoter variants can be studied with specific probes. It is widely used in chronobiology to study the temporal profile of expression of circadian genes and the effects of genetic manipulation on these oscillations. Methods to generate the riboprobes and to perform the RNase protection assay itself are described in this chapter.

Key Words

  • Circadian mRNA oscillations
  • RNase protection
  • alternative splicing
  • alternative promoters

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  • DOI: 10.1007/978-1-59745-257-1_24
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References

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  4. Emery, P., So, W. V., Kaneko, M., Hall, J. C., and Rosbash, M. (1998) CRY, a Drosophila clock and light-regulated cryptochrome, is a major contributor to circadian rhythm resetting and photosensitivity. Cell 95, 669–679.

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Author information

Authors and Affiliations

  1. Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA

    Patrick Emery

Authors
  1. Patrick Emery
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Editor information

Editors and Affiliations

  1. Department of Genetics, University of Leicester, Leicester, UK

    Ezio Rosato

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© 2007 Humana Press Inc.

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Emery, P. (2007). RNase Protection Assay. In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_24

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  • DOI: https://doi.org/10.1007/978-1-59745-257-1_24

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-417-3

  • Online ISBN: 978-1-59745-257-1

  • eBook Packages: Springer Protocols