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Northern Analysis of Sense and Antisense frequency RNA in Neurospora crassa

  • Cas Kramer
  • Susan K. Crosthwaite
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

In Northern analysis the presence of specific RNA transcripts is detected and their quantity can be estimated. RNA is separated using denaturing agarose gel electrophoresis and is subsequently transferred and fixed to a solid support, such as a nitrocellulose filter. When labeled probes are hybridized to these immobilized RNA molecules, their presence can be visualized by autoradiography. Here we describe Northern hybridization using radioactively labeled riboprobes to show circadian expression of endogenous sense and antisense frequency RNA in the filamentous fungus Neurospora crassa.

Key Words

Circadian filamentous fungus bread mold mycelium RNA sense antisense endogenous TRIzol blotting nitrocellulose riboprobe RNA probe 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Cas Kramer
    • 1
  • Susan K. Crosthwaite
    • 2
  1. 1.Department of GeneticsUniversity of LeicesterLeicesterUK
  2. 2.Faculty of Life SciencesUniversity of ManchesterManchesterUK

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