Extraction of Plant RNA

  • Michael G. Salter
  • Helen E. Conlon
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)


This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.

Key Words

Arabidopsis thaliana total RNA extraction acid phenol method efficient limited tissue 


  1. 1.
    Verwoerd, T. C., Dekker, B. M. M., and Hoekema, A. (1989) A small scale procedure for rapid isolation of plant RNA’s. Nucleic Acids Res. 17, 6, 2362.CrossRefGoogle Scholar
  2. 2.
    Salter, M. G., Franklin, K. A., and Whitelam, G. C. (2003) Gating of the rapid shade avoidance response by the circadian clock in plants. Nature 426, 680–683.CrossRefPubMedGoogle Scholar
  3. 3.
    Groppe, J. C., and Morse, D. E. (1993). Isolation of full length RNA templates for reverse transcription from tissues rich in RNAse and proteoglycans. Ann. Biochem. 210, 337–343.CrossRefGoogle Scholar
  4. 4.
    Perry, R. P., Torre, J. L., Kelly, D. E., and Greenberg, J. R. (1972) On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim. Biophys. Acta 14, 262 (2): 220–226.Google Scholar
  5. 5.
    Brawerman, G., Mendecki, J., and Lee, S. Y. (1972) A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry 11(4) 637–641.CrossRefPubMedGoogle Scholar
  6. 6.
    Ausubel, F. A., Brent, R., Kingston, R. E., et al. (1987) Current Protocols in Molecular Biology. John Wiley and Sons.Google Scholar
  7. 7.
    Wallace, D. M. (1987) Large-and small-scale phenol extractions. Methods Enzymol. 152, 33–41.CrossRefPubMedGoogle Scholar
  8. 8.
    Smabrook, J. and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Michael G. Salter
    • 1
  • Helen E. Conlon
    • 1
  1. 1.Department of BiologyUniversity of LeicesterLeicesterUK

Personalised recommendations