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Extraction of Plant RNA

  • Michael G. Salter
  • Helen E. Conlon
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.

Key Words

Arabidopsis thaliana total RNA extraction acid phenol method efficient limited tissue 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Michael G. Salter
    • 1
  • Helen E. Conlon
    • 1
  1. 1.Department of BiologyUniversity of LeicesterLeicesterUK

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