Extraction of Plant RNA
This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.
Key WordsArabidopsis thaliana total RNA extraction acid phenol method efficient limited tissue
- 4.Perry, R. P., Torre, J. L., Kelly, D. E., and Greenberg, J. R. (1972) On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim. Biophys. Acta 14, 262 (2): 220–226.Google Scholar
- 6.Ausubel, F. A., Brent, R., Kingston, R. E., et al. (1987) Current Protocols in Molecular Biology. John Wiley and Sons.Google Scholar
- 8.Smabrook, J. and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar