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RNA Extraction From Drosophila Heads

  • Patrick Emery
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

In Drosophila, input, pacemaker, and output genes are expressed circadianly. mRNA oscillations contribute largely to these rhythms. Determining RNA levels of circadian genes is thus frequently necessary to understand their regulation, or the effect of mutations and genetic manipulations on the function of the circadian pacemaker. RNA extraction is the prelude to several techniques aimed at measuring RNA levels. The procedure presented in this chapter is a rapid method to obtain a clean preparation of total RNA from fly heads that can be used for RNase protection, Northern blots. and real-time polymerase chain reaction (see  Chapters 23 25).

Key Words

mRNA extraction circadian mRNA oscillations RNAse protection Northern blots real-time PCR 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Patrick Emery
    • 1
  1. 1.Department of NeurobiologyUniversity of Massachusetts Medical SchoolWorcester

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