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Microarrays

Quality Control and Hybridization Protocol
  • Ken-ichiro Uno
  • Hiroki R. Ueda
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

Microarray technology is an exciting and promising tool, and is increasingly employed for studying circadian rhythms. To obtain optimal results from this technology, it is important to perform quality control experiments before engaging in genome-wide expression analysis. In this chapter, we provide an overview of quantitative polymerase chain reaction experiments using the ABI PRISM® 7900HT system for quality control of samples, and micorarray experiments using the Affymetrix GeneChip system for genome-wide expression analysis.

Key Words

Circadian rhythm quantitative PCR microarray quality control 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Ken-ichiro Uno
    • 1
  • Hiroki R. Ueda
    • 2
  1. 1.Functional Genomics Subunit, Center for Developmental BiologyRIKENKobe, HyogoJapan
  2. 2.Laboratory for Systems Biology, Center for Developmental BiologyRIKENKobe, HyogoJapan

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