Mutagenesis With Drosophila

  • Patrick Emery
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)


Forward genetics in Drosophila has profoundly affected our understanding of circadian rhythms in this organism and, more generally, in the animal kingdom. Most Drosophila pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies. An alkylating agent can be used to randomly alter the fly genome, or transposable elements can be mobilized to disrupt or increase the expression of the targeted genes. The advantages of these different methods are complementary.

Key Words

Forward genetics mutagenesis ethyl methane sulfonate P-element misexpression 


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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Patrick Emery
    • 1
  1. 1.Department of NeurobiologyUniversity of Massachusetts Medical SchoolWorcester

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