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Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes

  • Anthony Hall
  • Paul Brown
Part of the Methods in Molecular Biology™ book series (MIMB, volume 362)

Abstract

Arabidopsis thaliana is internationally adopted as the model plant species for molecular genetics. As such a huge range of resources are available for its study. Arabidopsis does not display any obvious circadian rhythms; however, rhythms in gene expression can be readily detected. For this reason the promoters of rhythmically expressed genes have been fused to the firefly luciferase gene. Using this reporter gene we have developed a number of automated techniques for monitoring luciferase activity in vivo in plants. This provides us with a robust and high-throughput assay for the circadian clock in Arabidopsis.

Key Words

Biological clock luciferase gene expression Arabidopsis plant transformation genetic engineering 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Anthony Hall
    • 1
  • Paul Brown
    • 2
  1. 1.School of Biological SciencesUniversity of LiverpoolLiverpoolUK
  2. 2.Interdisciplinary Programme for Cellular RegulationUniversity of WarwickCoventryUK

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