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Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in Cells

  • Mariana Brocardo
  • Beric R. Henderson
Part of the Methods in Molecular Biology™ book series (MIMB, volume 468)

Abstract

The adenomatous polyposis coli (APC) tumour suppressor gene is mutated in the majority of colon cancers. APC is a multi-domain protein whose distribution at different subcellular locations correlates with unique cellular processes. Our laboratory has focused on the link between APC subcellular location and function, and has characterized pathways for the trafficking of APC both into and out of the nucleus. Antibody specificity is an important factor in the determination of APC localization, and in this chapter we outline a strategy for the unambiguous detection of APC using a combination of biochemical and cell biology approaches.

Key words:

APC Western blotting Subcellular localization Colon cancer Immunofluorescence microscopy Antibodies APC detection Electrophoresis 

Notes

Acknowledgments

The authors thank the National Health and Medical Research Council of Australia and the Australian Research Council for funding support of this research.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Mariana Brocardo
    • 1
  • Beric R. Henderson
    • 1
  1. 1.Westmead Millennium InstituteWestmead, NSW, AustraliaWestmeadAustralia

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