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β-Catenin-Independent Wnt Pathways: Signals, Core Proteins, and Effectors

  • Protocol
Wnt Signaling

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 468))

Abstract

Wnt signaling activates several distinct intracellular pathways, which are important for cell proliferation, differentiation, and polarity. Wnt proteins are secreted molecules that typically signal across the membrane via interaction with the transmembrane receptor Frizzled. Following interaction with Frizzled, the downstream effect of the most widely studied Wnt pathway is stabilization and nuclear translocation of the cytosolic protein, β-catenin. In this chapter, we discuss two β-catenin-independent branches of Wnt signaling: 1) Wnt/planar cell polarity (PCP), a Wnt pathway that signals through the small GTPases, Rho and Rac, to promote changes in the actin cytoskeleton, and 2) Wnt/Ca2+, a Wnt pathway that promotes intracellular calcium transients and negatively regulates the Wnt/β-catenin pathway. Finally, during the course of our discussion, we highlight areas that require future research.

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Acknowledgments

This work was supported by the Howard Hughes Medical Institute and the National Institutes of Health (NIH) RO1 GM073887-03 to RTM.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Department of Pharmacology, and Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA, USA

    Richard G. James, William H. Conrad & Randall T. Moon

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  1. Richard G. James
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  2. William H. Conrad
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  3. Randall T. Moon
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  1. University of Melbourne, Parkville, Victoria, VIC 3010, Australia

    Elizabeth Vincan PhD

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James, R.G., Conrad, W.H., Moon, R.T. (2008). β-Catenin-Independent Wnt Pathways: Signals, Core Proteins, and Effectors. In: Vincan, E. (eds) Wnt Signaling. Methods in Molecular Biology™, vol 468. Humana Press. https://doi.org/10.1007/978-1-59745-249-6_10

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  • DOI: https://doi.org/10.1007/978-1-59745-249-6_10

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