RNA pp 87-105 | Cite as

Northern Blotting Analysis

Part of the Methods in Molecular Biology book series (MIMB, volume 703)

Abstract

Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments.

Key words

Gel electrophoresis northern blotting probe hybridization analysis mRNA 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.The Bartholin Institute, Copenhagen University HospitalCopenhagenDenmark
  2. 2.Department of Cellular and Molecular MedicineThe Panum Institute, University of CopenhagenCopenhagenDenmark

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