Volume 703 of the series Methods in Molecular Biology pp 87-105


Northern Blotting Analysis

  • Knud JosefsenAffiliated withThe Bartholin Institute, Copenhagen University Hospital Email author 
  • , Henrik NielsenAffiliated withDepartment of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen

* Final gross prices may vary according to local VAT.

Get Access


Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments.

Key words

Gel electrophoresis northern blotting probe hybridization analysis mRNA