Summary
Adipose tissue contains cell types other than adipocytes that may contribute to complications linked to obesity. For example, macrophages have been shown to infiltrate adipose tissue in response to a high-fat diet. Isolation of the stromal-vascular fraction of adipose tissue allows one to use flow cytometry to analyze cell surface markers on leukocytes. Here, we present a technical approach to identify subsets of leukocytes that differentially express cell surface markers.
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Acknowledgments
This work was supported by grants from the United States Department of Agriculture 6250-51000-046 (CWS). Partially supported through grant numbers DGE-0086397 and DGE-0440525 of the National Science Foundation (DKB).
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Brake, D.K., Smith, C.W. (2008). Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue. In: Yang, K. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 456. Humana Press. https://doi.org/10.1007/978-1-59745-245-8_16
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DOI: https://doi.org/10.1007/978-1-59745-245-8_16
Publisher Name: Humana Press
Print ISBN: 978-1-58829-916-1
Online ISBN: 978-1-59745-245-8
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