Metabolomics pp 141-155 | Cite as

Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics

Reversed-Phase Monolithic Capillary Chromatography and Hydrophilic Chromatography Coupled to Electrospray Ionization-Mass Spectrometry
  • Vladimir V. Tolstikov
  • Oliver Fiehn
  • Nobuo Tanaka
Part of the Methods in Molecular Biology™ book series (MIMB, volume 358)


Analysis of the entire metabolome as the sum of all detectable components in the sample rather than analysis of each individual metabolite is performed by the metabolomics approaches. To monitor in parallel hundreds or even thousands of metabolites, high-throughput techniques are required that enable screening for relative changes rather than absolute concentrations of compounds. Most analytical techniques for profiling small molecules consist of gas chromatography (GC) or high-performance liquid chromatography (HPLC) coupled to mass spectrometry. HPLC separations are better suited for the analysis of labile and high molecular weight compounds, and for the analysis of nonvolatile polar compounds in their natural form. Although GC- and HPLC-based profiling techniques are not truly quantitative, the compounds detecting and employing the acceptable standards may compare their relative amounts. We have demonstrated that reversed-phase monolithic capillary chromatography and hydrophilic chromatography can be successfully applied for sufficient plant crude extracts separations and metabolomics studies.


Theoretical Plate Monolithic Column Pump Flow Rate Capillary Monolithic Column Typical HILIC 
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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Vladimir V. Tolstikov
    • 1
  • Oliver Fiehn
    • 1
  • Nobuo Tanaka
    • 2
  1. 1.Genome CenterUniversity of California-DavisDavis
  2. 2.Department of Polymer Science and EngineeringKyoto Institute of TechnologyKyotoJapan

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