Abstract
Proteomic screening of complex biological samples becomes of increasing importance in clinical research and diagnosis. It is expected that the meager number of approx 35,000 human genes gives rise to more than 1,000,000 functional entities at the protein level. Thus, the proteome provides a much richer source of information than the genome for describing the state of health or disease of the human organism. Especially, the composition body fluids comprise a rich source of information on possible changes in the status of health or disease of particular organs and in consequence of the whole organism. Here we describe the application of capillary electrophoresis (CE) coupled on-line to an electrospray-ionization (ESI)-time-of-flight (TOF)-mass spectrometer) to the analysis of human urine for the identification of biomarkers for complications after allogeneic hematopoietic stem cell transplantation.
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References
Manabe, T. (1999) Capillary electrophoresis of proteins for proteomic studies. Electrophoresis 20, 3116–3121.
Oda, R. P., Clark, R., Katzmann, J. A., and Landers, J. P. (1997) Capillary electrophoresis as a clinical tool for the analysis of protein in serum and other body fluids. Electrophoresis 18, 1715–1723.
Jellum, E., Dollekamp, H., and Blessum, C. (1996) Capillary electrophoresis for clinical problem solving: analysis of urinary diagnostic metabolites and serum proteins. J. Chromatogr. Biomed. Appl. 683, 55–65.
Neususs, C., Pelzing, M., and Macht, M. (2002) A robust approach for the analysis of peptides in the low femtomole range by capillary electrophoresis-tandem mass spectrometry. Electrophoresis 23, 3149–3159.
Moini, M. (2004) Capillary electrophoresis-electrospray ionization mass spectrometry of amino acids, peptides, and proteins, in Capillary Electrophoresis of Proteins and Peptides (Strege, M. A. and Lagu A. L., eds.). Humana, Totowa, NJ: pp. 253–290.
Kaiser, T., Wittke, S., Krebs, R., Mischak, H., and Weissinger, E. M. (2004) Capillary Electrophoresis coupled to mass spectrometer for automated and robust polypetide recognition in body fluids for clinical use. Electrophoresis 25, 2044–2055
Kaiser T., Kamal H., Rank A., et al. (2004) Proteomics applied to clinical follow up of patients after allogeneic hematopoietic stem cell transplantation. Blood 104, 340–349.
Neuhoff, N., Kaiser, T., Wittke, S., et al. (2004) Mass spectrometry for the detection of differentially expressed proteins: a comparison of surface-enhanced laser desorption/ionization and capillary electrophoresis/mass spectrometry. Rapid Commun. Mass Spectrom. 18, 149–156.
Wittke, S., Mischak, H., Kolch, W., Raedler, T. J., and Wiedemann, K. (2005) Discovery of biomarkers in human urine and cerebrospinal fluid by capillary electrophoresis coupled mass spectrometry: towards new diagnostic and therapeutic approaches. Electrophoresis 26, 1476–1487.
Wittke, S., Fliser, D., Haubitz, M., et al. (2003) Determination of peptides and proteins in human urine with CE-MS—suitable tool for the establishment of new diagnostic markers. J. Chromatogr. A. 1013, 173–181.
Theodorescu, D., Fliser, D., Wittke, S., et al. (2005) Pilot study of capillary electrophoresis coupled to mass spectrometry as a tool to define potential prostate cancer biomarkers in urine. Electrophoresis 26, 2797–2808.
Johannesson, N., Wetterhall, M., Markides, K. E., and Bergquist, J. (2004) Monomer surface modifications for rapid peptide analysis by capillary electrophoresis and capillary electrochromatography coupled to electrospray ionization-mass spectrometry. Electrophoresis 25, 809–816.
Liu, C. Y. (2001) Stationary phases for capillary electrophoresis and capillary electrochromatography. Electrophoresis 22, 612–628.
Belder, D., Deege, A., Husmann, H., Kohler, F., and Ludwig, M. (2001) Cross-linked poly(vinylalcohol) as permanent hydrophilic column coating for capillary electrophoresis. Electrophoresis 22, 3813–3818.
Kolch, W., Neususs, C., Pelzing, M., and Mischak, H. (2005) Capillary electrophoresis—mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery. Mass Spectrometry Reviews 24, 959–977.
Ullsten, S., Zuberovic, A., Wetterhall, M., et al. (2004) A polyamine coating for enhanced capillary electrophoresis-electrospray ionization-mass spectrometry of proteins and peptides. Electrophoresis 25, 2090–2099.
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Weissinger, E.M., Mischak, H. (2007). Application of Proteomics to Posttransplantational Follow-Up. In: Beksac, M. (eds) Bone Marrow and Stem Cell Transplantation. Methods in Molecular Medicine, vol 134. Humana Press. https://doi.org/10.1007/978-1-59745-223-6_15
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DOI: https://doi.org/10.1007/978-1-59745-223-6_15
Publisher Name: Humana Press
Print ISBN: 978-1-58829-595-8
Online ISBN: 978-1-59745-223-6
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