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Mycobacteria Protocols pp 83–93Cite as

Whole Genome Analysis Using Microarrays

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 465))

Abstract

The development of microarray technology has allowed the genomes of mycobacteria to be directly compared to identify DNA regions that differ between strains due to deletion, insertion, or sequence divergence. The use of microarrays in comparative genomics has proved to be a valuable tool for comparing both mycobacterial species and strains. We describe here the methodology for comparing two mycobacterial DNA samples by microarray hybridization, from labeling and slide preparation, to DNA microarray analysis options. Further developments in microarray design and methodology promise to ensure that microarrays remain an important resource for comparative genomic studies in the future.

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Acknowledgments

S.J.W. was funded by an EU STREP Sixth Framework Programme Priority (LHP-CT-2004-012187). S.J.W., J.H., and P.D.B. would like to thank Kate Gould for her critical reading of the manuscript and acknowledge the Wellcome Trust and its Functional Genomics Resources Initiative for funding the multicollaborative microbial pathogen microarray facility at St. George’s (BμG@S).

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Authors and Affiliations

  1. Medical Microbiology, Division of Cellular & Molecular Medicine, St. George’s University of London, Cranmer Terrace, Tooting, London, SW17 0RE, UK

    S. J. Waddell PhD

  2. Division of Cellular and Molecular Medicine, St. George’s University of London, London, UK

    J. Hinds PhD & P.D. Butcher PhD

Authors
  1. S. J. Waddell PhD
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  2. J. Hinds PhD
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  3. P.D. Butcher PhD
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Corresponding author

Correspondence to S. J. Waddell PhD .

Editor information

Editors and Affiliations

  1. Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, Newark Street 4, London, E1 2AT, United Kingdom

    Tanya Parish

  2. Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, Newark Street 4, London, E1 2AT, United Kingdom

    Amanda Claire Brown

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Waddell, S.J., Hinds, J., Butcher, P. (2009). Whole Genome Analysis Using Microarrays. In: Parish, T., Brown, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 465. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-207-6_6

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  • DOI: https://doi.org/10.1007/978-1-59745-207-6_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-889-8

  • Online ISBN: 978-1-59745-207-6

  • eBook Packages: Springer Protocols

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