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Analyzing Lipid Metabolism: Activation and β-Oxidation of Fatty Acids

  • Paul Robert Wheeler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 465)

Abstract

There is massive gene replication predicted for the activation of fatty acids and their entry into the β-oxidation cycle for fatty acid oxidation. These two steps in fatty acid metabolism are catalyzed by FadD and FadE enzymes with 36 genes predicted for each of these respective activities in Mycobacterium tuberculosis. Here we present methods for the cell-free assay of types of enzymes in live bacteria, as well as for fatty acid oxidation overall.

Keywords

β-oxidation FadD FadE fatty-acid-CoA ligase (EC 6.2.1.3) fatty acid metabolism fatty acid oxidation fatty acyl-CoA dehydrogenase (EC 1.3.99.3) fatty acyl-CoA synthase (EC 6.2.1.3) mycobacteria Mycobacterium tuberculosis 

Notes

Acknowledgment

P.R.W. was supported through DEFRA funding.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Veterinary Laboratories Agency (Weybridge)Tuberculosis Research GroupSurreyUK

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