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Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization

  • Libin Shi
  • Jordi B. Torrelles
  • Delphi Chatterjee
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 465)

Abstract

In this chapter, we describe in detail the steps involved in isolation and characterization of lipoglycans from Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition, procedures involved in structural analysis such as immunoblotting with mAb CS-35 or CS-40, gas chromatography, gas chromatography/mass spectrometry, nuclear magnetic resonance spectroscopy, and endoarabinanase digestion followed by high-pH anion exchange chromatography and two-dimensional gel electrophoresis are presented.

Keywords

cell wall lipoarabinomannan (LAM) lipoglycans M. smegmatis M. tuberculosis Mycobacterium 

Notes

Acknowledgments

This work was supported by National Institutes of Health grant AI 37139 and in part from grant AI 52439.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Libin Shi
    • 1
  • Jordi B. Torrelles
    • 2
  • Delphi Chatterjee
    • 3
  1. 1.Department of Microbiology, Immunology, and PathologyColorado State UniversityFort CollinsUSA
  2. 2.Department of Internal Medicine and Division of Infectious DiseasesThe Ohio State UniversityColumbusUSA
  3. 3.Department of Microbiology, Immunology, and Pathology, 1682 Campus DeliveryColorado State UniversityFort CollinsUSA

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