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Mycobacteria Protocols pp 217–228Cite as

Ins and Outs of Mycobacterial Plasmids

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 465))

Abstract

The importance of plasmids for molecular research cannot be underestimated. These double-stranded DNA units that replicate independently of the chromosomal DNA are as valuable to bacterial geneticists as a carpenter’s hammer. Fortunately, today the mycobacterial research community has a number of these genetic tools at its disposal, and the development of these tools has greatly accelerated the study of mycobacterial pathogens. However, working with mycobacterial cloning plasmids is still not always as straightforward as working with Escherichia coli plasmids, and therefore a number of precautions and potential pitfalls will be discussed in this chapter.

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Author information

Authors and Affiliations

  1. Institute for Tuberculosis Research, College of Pharmacy, Rm 412, University of Illinois at Chicago, 833 S. Wood St., Chicago, Illinois, USA, 60612-7231

    Farahnaz Movahedzadeh PhD

  2. Medical Microbiology, VU University Medical Centre, Amsterdam, The Netherlands

    Wilbert Bitter PhD

Authors
  1. Farahnaz Movahedzadeh PhD
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  2. Wilbert Bitter PhD
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Correspondence to Farahnaz Movahedzadeh PhD .

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Editors and Affiliations

  1. Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, Newark Street 4, London, E1 2AT, United Kingdom

    Tanya Parish

  2. Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, Newark Street 4, London, E1 2AT, United Kingdom

    Amanda Claire Brown

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Movahedzadeh, F., Bitter, W. (2009). Ins and Outs of Mycobacterial Plasmids. In: Parish, T., Brown, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 465. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-207-6_14

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  • DOI: https://doi.org/10.1007/978-1-59745-207-6_14

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  • Publisher Name: Humana Press, Totowa, NJ

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