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Microchip Capillary Electrophoresis

Application to Peptide Analysis

  • Protocol
The Protein Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Peptides are a large class of molecules that differ in size, charge, conformation, hydrophobiCity>, and the ability to form biospecific complexes. Peptides play an important role in many physiological processes, including the regulation of pain, blood pressure, and immune response, and can act as antibiotics, coenzymes/enzyme inhibitors, drugs, growth stimulators, hormones, neurotransmitters, and toxins (1).

Since the identification of leu-enkephalin in the 1970s, the role of neuropeptides in the regulation of the central nervous system and signal transduction has been extensively investigated (1). Identification of specific peptides and their functions may permit the design of pharmacologically active synthetic analogs that could be used for the treatment of neurological diseases such as Parkinson’s and Alzheimer’s. Peptide analysis is also important for proteomics research. Digestion of a protein yields distinct peptide mixtures, which are separated and mapped in order to characterize the parent molecule. In some cases, multidimensional separations are needed to achieve resolution and identification of all peptide components.

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Author information

Authors and Affiliations

  1. Life Sciences Interface, Tyndall National Institute, Cork, Ireland

    Barbara A. Fogarty

  2. Pfizer Research, St. Louis, MO, USA

    Nathan A. Lacher

  3. Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA

    Susan M. Lunte

Authors
  1. Barbara A. Fogarty
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  2. Nathan A. Lacher
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  3. Susan M. Lunte
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Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Hertfordshire, Hatfield, Hertfordshire, UK

    John M. Walker

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Fogarty, B.A., Lacher, N.A., Lunte, S.M. (2009). Microchip Capillary Electrophoresis. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_37

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  • DOI: https://doi.org/10.1007/978-1-59745-198-7_37

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-474-6

  • Online ISBN: 978-1-59745-198-7

  • eBook Packages: Springer Protocols

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