Abstract
The fusion of antigen-primed B cells with transformed myeloma cells results in immortalized hybridomas that secrete antibodies. The subsequent cloning of the hybridomas gives rise to cell lines secreting monoclonal antibodies (MAbs) of a single specificity (1). This technology has had a tremendous impact on research and medicine, with MAbs being used to identify and characterize the biological significance of myriads of molecules. The outcome has been the development of diagnostic tests and therapies for the detection and management of disease (2). The amount and purity of a MAb required for any given purpose can vary greatly. Here we describe the procedures involved in the maintenance and management of hybridomas and suggest techniques for maximising yields.
Keywords
- Complete Medium
- Production Module
- Tissue Culture Flask
- Cell Bank
- Corning Incorporate
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Acknowledgments
The authors thank Joachim Lücke for permission to reproduce the miniPERM images. GE, CJ and DD are supported by the Scottish Executive Environment and Rural Affairs Department (SEERAD). This work was conducted in conjunction with the BBSRC/ SEERAD Toolbox.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Entrican, G., Jepson, C., Deane, D. (2009). Growing Hybridomas. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_200
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DOI: https://doi.org/10.1007/978-1-59745-198-7_200
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60327-474-6
Online ISBN: 978-1-59745-198-7
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