Advertisement

Purification of IgG by Precipitation with Polyethylene Glycol (PEG)

  • Mark Page
  • Robin Thorpe
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

PEG precipitation works well for IgM, but is less efficient for IgG; salt precipitation methods are usually recommended for IgG. PEG precipitation may be preferred in multistep purifications that use ion-exchange columns, because the ionic strength of the Ig is not altered. Furthermore, it is a very mild procedure that usually results in little denaturation of antibody. This procedure is applicable to both polyclonal antisera and most MAb containing fluids.

Keywords

Ionic Strength Ammonium Sulfate Polyethylene Glycol Precipitation Method Sulfate Precipitation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 1
  1. 1.Division of ImmunobiologyNational Institute for Biological Standards and ControlPotters BarUK

Personalised recommendations