Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification

  • Minoru Takata
Part of the Methods in Molecular Biology™ book series (MIMB, volume 439)


With the increasing knowledge on the genetic alterations associated with various cancers, molecular analysis of these alterations on formalin-fixed paraffin-embedded tissue is of increasing importance. Multiplex ligation-dependent probe amplification (MLPA) is a novel technique to measure the copy number of up to 45 nucleic acid sequences in a single reaction. This method relies on sequence-specific probe hybridization to genomic DNA followed by multiplex-PCR amplification of the hybridized probe, and semi-quantitative analysis of the resulting PCR products. This method is easy to perform; requiring as little as 50 ng of DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissue.


Chromosome aberrations FFPE tissue DNA imbalances melanocytic neoplasm 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Minoru Takata
    • 1
  1. 1.Shinshu University School of MedicineMatsumotoJapan

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