Coimmunoprecipitation and Proteomic Analyses
Defining protein-protein interaction networks is a major goal of proteomics. Here, we present a protocol for coimmunoprecipitation, a technique suitable for the isolation of whole protein complexes in vivo and their subsequent identification by either immunoblotting or mass spectrometric sequencing combined to database search.
Keywordscoimmunoprecipitation immunoblotting protein complexes protein-protein interaction mass spectrometry
This work was supported by the Austrian Genome Network GEN-AU/ APP-II of the Austrian Ministry for Education, Science and Culture and by the OeNB (Pr.Nr. 10855).
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