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Coimmunoprecipitation and Proteomic Analyses

  • S. Fabio Falsone
  • Bernd Gesslbauer
  • Andreas J. Kungl
Part of the Methods in Molecular Biology™ book series (MIMB, volume 439)

Abstract

Defining protein-protein interaction networks is a major goal of proteomics. Here, we present a protocol for coimmunoprecipitation, a technique suitable for the isolation of whole protein complexes in vivo and their subsequent identification by either immunoblotting or mass spectrometric sequencing combined to database search.

Keywords

coimmunoprecipitation immunoblotting protein complexes protein-protein interaction mass spectrometry 

Notes

Acknowledgments

This work was supported by the Austrian Genome Network GEN-AU/ APP-II of the Austrian Ministry for Education, Science and Culture and by the OeNB (Pr.Nr. 10855).

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • S. Fabio Falsone
    • 1
  • Bernd Gesslbauer
    • 1
  • Andreas J. Kungl
    • 1
  1. 1.Institute of Pharmaceutical SciencesUniversity of GrazGrazAustria

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