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Scanning for DNA Variants by Denaturant Capillary Electrophoresis

  • Per O. Ekstrøm
Part of the Methods in Molecular Biology™ book series (MIMB, volume 439)

Abstract

Analysis and detection of DNA variation is important in any field of biology. Hence, numerous methods have been developed to analyze DNA. A simple yet effective way of analyzing DNA is by denaturant capillary electrophoresis (DCE). The method is in theory applicable to 95% of the human genome. The method involves three steps; fragment design, PCR amplification and allele separation. The allele separation can in principle be performed with any DNA capillary sequencing instrument.

Keywords

DGGE (denaturant gradient gel electrophoresis) CDCE (constant denaturant capillary electrophoresis) CTCE (cycling temperature capillary electrophoresis) PCR (polymerase chain reaction) dsDNA — double strand DNA ssDNA — single strand DNA 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Per O. Ekstrøm
    • 1
  1. 1.The Norwegian Radium HospitalOsloNorway

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