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Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors

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HIV Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 485))

Abstract

Cellular proteins are critically involved in all steps of the human immunodeficiency virus type 1 HIV-1) life cycle. Disruption of host functions essential for virus replication or discovery of new proteins that block viral replication may provide novel antiviral approaches. In recent years, genetic selections for and against genes carried by retroviral vectors have become increasingly powerful, allowing for the isolation of cells with altered susceptibility to virus infection. Screening complementary DNA libraries for clones able to induce resistance to infection by recombinant HIV-1 genomes, has proved to be an excellent tool to identify new interfering factors. The restriction factors TRIM5α1, the Zinc Finger Antiviral Protein ZAP) 2 as well as the dominant negative factor N-86-HnRNPU 3 have all been discovered by means of such genetic screens. Here we report the strategy and techniques to prepare a library and isolate HIV antiviral genes, using the identification of {N-86-HnRNPU} as an example.

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Valente, S.T., Goff, S.P. (2009). Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols. Methods In Molecular Biology™, vol 485. Humana Press. https://doi.org/10.1007/978-1-59745-170-3_17

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  • DOI: https://doi.org/10.1007/978-1-59745-170-3_17

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-859-1

  • Online ISBN: 978-1-59745-170-3

  • eBook Packages: Springer Protocols

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