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Measuring Apoptosis in Neural Stem Cells

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Neural Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 438))

Summary

In trauma to, and diseases of, the central nervous system (CNS), apoptotic events are frequently observed in and around areas of damage. Human embryonic stem cells (hESCs) and their progeny have been suggested as possible therapeutic agents in the treatment of CNS diseases. The success of stem cell transplantation not only depends on the capacity of these cells to retain their functionality after transplant into the CNS but also on their ability to resist the in situ environmental cues that may lead to apoptosis. Although there are many methods used to detect apoptosis, the assessment of apoptosis in adherent cultures of primary stem cells and their progeny is more limited. We describe a series of protocols we have used to assess apoptosis in these cells.

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Author information

Authors and Affiliations

  1. Department of Neurology, Keck School of Medicine of the University of Southern California, Los Angeles, CA

    Brett T. Lund PhD

  2. Department of Cell and Neurobiology, Keck School of Medicine of the University of Southern California, Los Angeles, CA

    Eve E. Kelland PhD

  3. Department of Cell and Neurobiology, Zilkha Neurogenetic Research Institute, Los Angeles, CA

    Eve E. Kelland PhD

Authors
  1. Brett T. Lund PhD
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  2. Eve E. Kelland PhD
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Editors and Affiliations

  1. Keck School of Medicine, University of Southern California, 2025 Zonal Ave., Los Angeles, CA 90033, USA

    Leslie P. Weiner

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Lund, B.T., Kelland, E.E. (2008). Measuring Apoptosis in Neural Stem Cells. In: Weiner, L.P. (eds) Neural Stem Cells. Methods in Molecular Biology™, vol 438. Humana Press. https://doi.org/10.1007/978-1-59745-133-8_19

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  • DOI: https://doi.org/10.1007/978-1-59745-133-8_19

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-846-1

  • Online ISBN: 978-1-59745-133-8

  • eBook Packages: Springer Protocols

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