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Osteoporosis pp 323-334 | Cite as

Determination of Bone's Mechanical Matrix Properties by Nanoindentation

Protocol
Part of the Methods In Molecular Biology™ book series (MIMB, volume 455)

Abstract

Osteoporosis is a devastating disease that is characterized not only by a reduction in bone quantity but also by deterioration in bone quality. The quality of bone tissue is greatly influenced by its mechanical properties and, therefore, investigations into the etiology and enhanced detection of osteoporosis, or the efficacy of interventions, may require the assessment of bone's mechanical properties at the level of the tissue. Nanoindentation is a relatively new technique that is capable of evaluating bone's quasi-static and dynamic mechanical properties on extremely small volumes of tissue. These data can be used directly to describe the pre-yield properties of the matrix, but can also be combined with imaging techniques and mechanical models to extrapolate the mechanical properties from the level of the tissue to that of the organ.

Keywords

Nanoindentation mechanical properties bone matrix stiffness elastic modulus hardness trabecular bone cortical bone loading rate 
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Notes

Acknowledgments

The authors thank Jim Quinn for assistance with acquiring the SEM image. Financial support by NASA NAG 9-1499 (S.J.), the Wallace Coulter Foundation (S.J.), the Whitaker Foundation RG-02-0564 (S.J.), the National Space Biomedical Research Institute (TD00207 & TD00405) through NASA Cooperative Agreement NCC 9-58 (Y.Q.), NIH (R01 AR49286 Y.Q, R01 AR49694) Y.Q, and R01 AR052778, S.J), and the US Army Medical Research and Material Command (DAMD-17-02-1-0218) (Y.Q.) is gratefully acknowledged.

References

  1. 1.
    1. Hans, D., Fuerst, T., Lang, T., et al. (1997) How can we measure bone quality? Baillieres Clin Rheumatol 11, 495–515.CrossRefPubMedGoogle Scholar
  2. 2.
    2. Riggs, B. L., Hodgson, S. F., O'Fallon, W. M., et al. (1990) Effect of fluoride treatment on the fracture rate in postmenopausal women with osteoporosis. NEJM 322, 802–809.CrossRefPubMedGoogle Scholar
  3. 3.
    3. Mashiba, T., Hirano, T., Turner, C. H., et al. (2000) Suppressed bone turnover by bisphospho-nates increases microdamage accumulation and reduces some biomechanical properties in dog rib. J Bone Miner Res 15, 613–620.CrossRefPubMedGoogle Scholar
  4. 4.
    4. Judex, S., Boyd, S. K., Qin, Y. X., et al. (2003) Combining high-resolution microct with material composition to define the quality of bone tissue. Curr Osteoporosis Repts 1, 11–19.CrossRefGoogle Scholar
  5. 5.
    5. Mittra, E., Rubin, C., Qin, Y. X. (2005) Interrelationship of trabecular mechanical and micro-structural properties in sheep trabecular bone. J Biomech 38, 1229–1237.CrossRefPubMedGoogle Scholar
  6. 6.
    6. Judex, S., Boyd, S., Qin, Y. X., et al. (2003) Adaptations of trabecular bone to low magnitude vibrations result in more uniform stress and strain under load. Ann Biomed Eng 31, 12–20.CrossRefPubMedGoogle Scholar
  7. 7.
    7. Keaveny, T. M., Borchers, R. E., Gibson, L. J., et al. (1993) Trabecular bone modulus and strength can depend on specimen geometry. J Biomech 26, 991–1000.CrossRefPubMedGoogle Scholar
  8. 8.
    8. Silva, M. J., Brodt, M. D., Fan, Z., et al. (2004) Nanoindentation and whole-bone bending estimates of material properties in bones from the senescence accelerated mouse SAMP6. J Biomech 37, 1639–1646.CrossRefPubMedGoogle Scholar
  9. 9.
    9. Wang, X., Shanbhag, A. S., Rubash, H. E., et al. (1999) Short-term effects of bisphosphonates on the biomechanical properties of canine bone. J Biomed Mater Res 44, 456–460.CrossRefPubMedGoogle Scholar
  10. 10.
    10. Reilly, G. C., Currey, J. D. (2000) The effects of damage and microcracking on the impact strength of bone. J Biomech 33, 337–343.CrossRefPubMedGoogle Scholar
  11. 11.
    11. Tanck, E., Van Donkelaar, C. C., Jepsen, K. J., et al. (2004) The mechanical consequences of mineralization in embryonic bone. Bone 35, 186–190.CrossRefPubMedGoogle Scholar
  12. 12.
    12. Oliver, W. C., Pharr, G. M. (1992) An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments. J Mater Res 7, 1564–1583.CrossRefGoogle Scholar
  13. 13.
    13. Fan, Z., Rho, J. Y. (2003) Effects of viscoelasticity and time-dependent plasticity on nanoin-dentation measurements of human cortical bone.J Biomed Mater Res A 67, 208–214.CrossRefGoogle Scholar
  14. 14.
    14. Rho, J. Y., Tsui, T. Y. , Pharr, G. M. (1997) Elastic properties of human cortical and trabecular lamellar bone measured by nanoindentation. Biomaterials 18, 1325–1330.CrossRefPubMedGoogle Scholar
  15. 15.
    15. Keaveny, T. M., Hayes, W. C. (1993) A 20-year perspective on the mechanical properties of trabecular bone. J Biomech Eng 115, 534–542.CrossRefPubMedGoogle Scholar
  16. 16.
    16. Roy, M. E., Rho, J. Y. , Tsui, T. Y. , et al. (1999) Mechanical and morphological variation of the human lumbar vertebral cortical and trabecular bone. J Biomed Mater Res 44, 191–197.CrossRefPubMedGoogle Scholar
  17. 17.
    17. Turner, C. H., Rho, J., Takano, Y. , et al. (1999) The elastic properties of trabecular and cortical bone tissues are similar: results from two microscopic measurement techniques. J Biomech 32, 437–441.CrossRefPubMedGoogle Scholar
  18. 18.
    18. Busa, B., Miller, L. M., Rubin, C. T., et al. (2005) Rapid establishment of chemical and mechanical properties during lamellar bone formation. Calcif Tissue Int 77, 386–394.CrossRefPubMedGoogle Scholar
  19. 19.
    19. Zysset, P. K., Guo, X. E., Hoffler, C. E., et al. (1999) Elastic modulus and hardness of cortical and trabecular bone lamellae measured by nanoindentation in the human femur. J Biomech 32, 1005–1012.CrossRefPubMedGoogle Scholar
  20. 20.
    20. Iwamoto, J., Yeh, J. K., Aloia, J. F. (1999) Differential effect of treadmill exercise on three cancellous bone sites in the young growing rat. Bone 24, 163–169.CrossRefPubMedGoogle Scholar
  21. 21.
    21. Xia, Y. , Lin, W., Qin, Y. X. (2005) The influence of cortical end-plate on broadband ultrasound attenuation measurements at the human calcaneus using scanning confocal ultrasound. J Acoust Soc Am 118, 1801–1807.CrossRefPubMedGoogle Scholar
  22. 22.
    22. Rho, J. Y. , Zioupos, P., Currey, J. D., et al. (1999) Variations in the individual thick lamellar properties within osteons by nanoindentation. Bone 25, 295–300.CrossRefPubMedGoogle Scholar
  23. 23.
    23. Fan, Z., Swadener, J. G., Rho, J. Y. , et al. (2002) Anisotropic properties of human tibial cortical bone as measured by nanoindentation. J Orthop Res 20, 806–810.CrossRefPubMedGoogle Scholar
  24. 24.
    24. Hoffler, C. E., Guo, X. E., Zysset, P. K., et al. (2005) An application of nanoindentation technique to measure bone tissue Lamellae properties. J Biomech Eng 127, 1046–1053.CrossRefPubMedGoogle Scholar
  25. 25.
    25. Hengsberger, S., Kulik, A., Zysset, P. (2002) Nanoindentation discriminates the elastic properties of individual human bone lamellae under dry and physiological conditions. Bone 30, 178–184.CrossRefPubMedGoogle Scholar
  26. 26.
    26. Mittra, E., Akella, S., Qin, Y. X. (2006) The effects of embedding material, loading rate and magnitude, and penetration depth in nanoindentation of trabecular bone. J Biomed Mater Res A 79, 86–93.CrossRefGoogle Scholar
  27. 27.
    27. Carter, D. R., Hayes, W. C. (1976) Bone compressive strength: the influence of density and strain rate. Science 194, 1174–1176.CrossRefPubMedGoogle Scholar
  28. 28.
    28. Sasaki, N., Nakayama, Y. , Yoshikawa, M., et al. (1993) Stress relaxation function of bone and bone collagen. J Biomech 26, 1369–1376.CrossRefPubMedGoogle Scholar
  29. 29.
    29. Yamashita, J., Li, X., Furman, B. R., et al. (2002) Collagen and bone viscoelasticity: a dynamic mechanical analysis. J Biomed Mater Res 63, 31–36.CrossRefPubMedGoogle Scholar
  30. 30.
    Loubet, J. L. (1996) Some measurements of viscoelastic properties with the help of nanoin-dentation. NIST Spec Pub 31–4.Google Scholar
  31. 31.
    31. Donnelly, E., Baker, S. P., Boskey, A. L., et al. (2006) Effects of surface roughness and maximum load on the mechanical properties of cancellous bone measured by nanoindentation. J Biomed Mater Res A 77, 426–435.CrossRefGoogle Scholar
  32. 32.
    32. Currey, J. D. (1990) Physical characteristics affecting the tensile failure properties of compact bone. J Biomech 23, 83–844.CrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of Biomedical EngineeringState University of New York at Stony BrookStony BrookUSA

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