Abstract
Host factors are crucial determinants of viral pathogenicity. Identifying host factors and their contributions to virus infections may lead to the development of novel antiviral strategies. The recently developed virus-induced gene silencing (VIGS) approach offers a rapid means to knock down expression of a given gene in plants. VIGS can be used to determine biological function of candidate genes or to discover new genes that play a role in a given biological pathway. Here, we describe genome-wide Tobacco rattle virus (TRV)-based VIGS screening methods to identify host factors involved in viral pathogenicity.
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Acknowledgments
We thank Tessa Burch-Smith for critical reading of the manuscript and Yule Liu for helpful comments. VIGS work in S.P.D.-K. laboratory is supported by National Science Foundation Plant Genome Grant DBI-0211872.
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Zhu, X., Dinesh-Kumar, S.P. (2008). Virus-Induced Gene Silencing as a Tool to Identify Host Genes Affecting Viral Pathogenicity. In: Foster, G.D., Johansen, I.E., Hong, Y., Nagy, P.D. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 451. Humana Press. https://doi.org/10.1007/978-1-59745-102-4_43
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DOI: https://doi.org/10.1007/978-1-59745-102-4_43
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