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Plant Virology Protocols pp 3–19Cite as

Plant–Virus Interactions

  • Protocol

Part of the Methods in Molecular Biology™ book series (MIMB,volume 451)

Abstract

A variety of techniques have been used to examine plant viral genomes, the functions of virus-encoded proteins, plant responses induced by virus infection and plant–virus interactions. This overview considers these technologies and how they have been used to identify novel viral and plant proteins or genes involved in disease and resistance responses, as well as defense signaling. These approaches include analysis of spatial and temporal responses by plants to infection, and techniques that allow the expression of viral genes transiently or transgenically in planta, the expression of plant and foreign genes from virus vectors, the silencing of plants genes, imaging of live, infected cells, and the detection of interactions between viral proteins and plant gene products, both in planta and in various in vitro or in vivo systems. These methods and some of the discoveries made using these approaches are discussed.

Keywords

  • Agroinfection
  • Agroinfiltration
  • Green fluorescent protein
  • Plant gene isolation
  • Microarrays
  • PR proteins
  • Resistance responses
  • Salicylic acid
  • Transgenic plants
  • Virus-induced gene silencing

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Acknowledgments

JES acknowledges support from the U.S. Department of Agriculture/National Research Initiative Competitive Grant No. 2003–35319–13778. PP was supported by a grant-in-aid from the Scottish Executive Environment and Rural Affairs Department to the SCRI.

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Authors and Affiliations

  1. Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

    Peter Palukaitis

  2. Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK

    John P. Carr

  3. Division of Plant Sciences, 108 Waters Hall, University of Missouri, Colombia, MO 65211, USA

    James E. Schoelz

Authors
  1. Peter Palukaitis
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  2. John P. Carr
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  3. James E. Schoelz
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Editor information

Editors and Affiliations

  1. University of Bristol, Bristol, UK

    Gary D. Foster

  2. University of Aarhus, Aarhus, Denmark

    I. Elisabeth Johansen

  3. University of Warwick, Warwick, UK

    Yiguo Hong

  4. University of Kentucky, Kentucky, USA

    Peter D. Nagy

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© 2008 Humana Press, a part of Springer Science + Business Media, LLC

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Palukaitis, P., Carr, J.P., Schoelz, J.E. (2008). Plant–Virus Interactions. In: Foster, G.D., Johansen, I.E., Hong, Y., Nagy, P.D. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 451. Humana Press. https://doi.org/10.1007/978-1-59745-102-4_1

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  • DOI: https://doi.org/10.1007/978-1-59745-102-4_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-827-0

  • Online ISBN: 978-1-59745-102-4

  • eBook Packages: Springer Protocols

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