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Detection of siRNAs and miRNAs

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 451)

Abstract

Small RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) play crucial roles in establishing general host defense mechanisms against viral infections in plants and the development of disease symptoms. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. However, because of the small size of miRNAs and siRNAs, their detection is technically demanding. Here, we describe methods for robust and sensitive detection of small RNAs by Northern blot analysis and in situ hybridization.

Keywords

siRNA miRNA Detection Northern blot In situ hybridization LNA 
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Notes

Acknowledgements

Z. H. was supported by Hungarian Scientific Research Fund (OTKA; K61461) and Bolyai Janos Fellowship.

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  1. 1.Wilhelm Johannsen Center for Functional Genome Research, Department of Cellular and Molecular MedicineUniversity of CopenhagenHoersholmDenmark
  2. 2.Agricultural Biotechnology CenterPlant Biology InstituteGödöllöHungary

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