Summary
Virus indexing of seed potatoes can be carried out by growing eye plugs to produce small plants and then testing them by ELISA, but this method is time consuming. Direct testing of the eye plugs by ELISA is not reliable, and so a method has been developed for the routine testing of seed potatoes for virus by PCR.
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Acknowledgements
The authors would like to thank the British Potato Council and Plant Health Division of Defra for initial funding of the development work.
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Boonham, N., Laurenson, L., Weekes, R., Mumford, R. (2009). Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR. In: Burns, R. (eds) Plant Pathology. Methods in Molecular Biology, vol 508. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-062-1_19
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DOI: https://doi.org/10.1007/978-1-59745-062-1_19
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Publisher Name: Humana Press, Totowa, NJ
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