Summary
The mitochondrial oxidative phosphorylation involves five multimeric complexes imbedded in the inner membrane: complex I (Nicotinamide Adenine Dinucleotide (NADH) quinone oxidoreductase), II (succinate dehydrogenase), III (ubiquinol cytochrome c oxido reductase or bc1 complex), IV (cytochrome c oxidase), and V (ATP synthase). These respiratory complexes are conserved from the yeast Saccharomyces cerevisiae to human with the exception of complex I, which is replaced by three NADH dehydrogenases in S. cerevisiae. Here, we provide several protocols allowing an exhaustive characterization of each yeast complex: this chapter describes procedures from mitochondria preparation to measurement of the activity of each complex and analysis of their subunit composition and provides information on the interactions between different complexes.
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Acknowledgments
We thank N. Lachacinski and S. Marsy for excellent technical assistance; Drs T.D. Fox and J. Velours for antibodies; and Dr. P. Hamel for critical reading of the manuscript and for looking over the English. This work was supported by grants from the “Association Franccaise contre les Myopathies” and from the C.N.R.S. program “Prot’eomique et G’enie des Prot’eines”.
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Lemaire, C., Dujardin, G. (2008). Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria. In: Pflieger, D., Rossier, J. (eds) Organelle Proteomics. Methods in Molecular Biology™, vol 432. Humana Press. https://doi.org/10.1007/978-1-59745-028-7_5
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DOI: https://doi.org/10.1007/978-1-59745-028-7_5
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