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High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling

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Glycomics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 534))

Summary

Sensitive and fast methods for the profiling of biologically important molecules are highly demanded. Mucins are densely O-glycosylated glycoproteins found at mucosal surfaces and are of great medical interest. Here we describe sensitive methods for the analysis of O-glycans from mucins using gel electrophoresis, and chromatography by nanoLC on graphite columns and structural analysis by electrospray mass spectrometry on a linear trap mass spectrometer.

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References

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Acknowledgements

The work was supported by the Swedish Research Council (No. 7491 and an expensive equipment grant) and IngaBritt and Arne Lundberg Foundation.

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Authors and Affiliations

  1. Department of Medical Biochemistry and Cell Biology, Göteborg University, Box 440, 405 30, Gothenburg, Sweden

    Gunnar C. Hansson

Authors
  1. Hasse Karlsson
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  2. Jessica M. Holmén Larsson
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  3. Kristina A. Thomsson
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  4. Iris Härd
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  5. Malin Bäckström
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  6. Gunnar C. Hansson
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Corresponding author

Correspondence to Gunnar C. Hansson .

Editor information

Editors and Affiliations

  1. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia

    Nicolle H. Packer

  2. Centre for Bioanalytical Science, Chemistry Department, NUI Galway, Galway, Ireland

    Niclas G. Karlsson

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Karlsson, H., Larsson, J.M.H., Thomsson, K.A., Härd, I., Bäckström, M., Hansson, G.C. (2009). High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling. In: Packer, N.H., Karlsson, N.G. (eds) Glycomics. Methods in Molecular Biology™, vol 534. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-022-5_9

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  • DOI: https://doi.org/10.1007/978-1-59745-022-5_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-774-7

  • Online ISBN: 978-1-59745-022-5

  • eBook Packages: Springer Protocols

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