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Glycomics pp 133–145Cite as

Collision-Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 534))

Summary

The complexity of glycans poses a major challenge for structure elucidation. Tandem mass spectrometry is currently an efficient and powerful technique for the structural characterization of glycans. Collision-induced dissociation (CID) is most commonly used, and involves first isolating the glycan ions of interest, translationally exciting them, and then striking them with inert target gas to fragment the precursor ions. The structural information of the glycan can be obtained from the fragment ions of the tandem MS spectra.

In this chapter, sustained off-resonance irradiation-collision-induced dissociation (SORI-CID) implemented with matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT ICR MS) is demonstrated to be a useful analysis tool for structural elucidation of mucin-type O-glycans released from mucin glycoproteins. The mechanisms by which the glycans undergo fragmentations in the tandem mass analysis are also discussed.

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Acknowledgments

We gratefully acknowledge the financial support by the National Institutes of Health (R01 GM049077). We also wish to thank Dr. Jinhua Zhang for her kind help and useful suggestions.

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Authors and Affiliations

  1. Department of Chemistry, University of California, Davis, CA, 95616, USA

    Carlito B. Lebrilla

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  1. Bensheng Li
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  2. Hyun Joo An
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  3. Jerry L. Hedrick
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  4. Carlito B. Lebrilla
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Corresponding author

Correspondence to Carlito B. Lebrilla .

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Editors and Affiliations

  1. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia

    Nicolle H. Packer

  2. Centre for Bioanalytical Science, Chemistry Department, NUI Galway, Galway, Ireland

    Niclas G. Karlsson

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Li, B., An, H.J., Hedrick, J.L., Lebrilla, C.B. (2009). Collision-Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans. In: Packer, N.H., Karlsson, N.G. (eds) Glycomics. Methods in Molecular Biology™, vol 534. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-022-5_10

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  • DOI: https://doi.org/10.1007/978-1-59745-022-5_10

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