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Microarray Image Scanning

  • Latha Ramdas
  • Wei Zhang
Part of the Methods in Molecular Biology™ book series (MIMB, volume 319)

Abstract

Of the technologies available for measuring gene expression, microarrays using cDNA targets is one of the most common and well-developed high-throughput techniques. With this technique, the expression levels of thousands of genes are measured simultaneously. DNA probes are immobilized on solid surfaces, either membrane-based or chemically coated glass surfaces. On glass arrays, the probes are hybridized with fluorescent-labeled target samples. Fluorescence intensities, which reflect gene expression levels, are detected by imaging the array using a laser or white-light source and capturing the image using photomultiplier tube detection or a charge-coupled device camera. Different laser-based scanners are used in laboratories to scan microarray images. This chapter discusses the imaging process and the protocols being developed.

Key Words

Genomics microarray laser scanners imaging gene expression fluorescence 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Latha Ramdas
    • 1
  • Wei Zhang
    • 1
  1. 1.Cancer Genomics Core LaboratoryUniversity of Texas M.D. Anderson cancer CenterHouston

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