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Molecular Biomethods Handbook pp 89–93Cite as

Northern Blot Analysis

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Abstract

The analysis of RNA is central to a wide range of molecular-biology studies, because It 1s often important to obtain informatlon about the expression of genes in hving organlsms Filter hybrldlzatlon of size-separated RNAs with a labeled nucleic-acid probe, a techinque known as northern-blot analysis, is designed to address the study of RNA sequences 1 This procedure, which derives from Southern-blot analysis (see Chapter 8 in this volume), is based on the ability of complementary single-stranded nucleic acids to form hybrid molecules 2 In brief, RNA is separated according to size on an agarose gel under denaturing condltlons, transferred (blotted) and lrreverslbly bound to a nylon or introcellulose membrane, and hybridized with a 32P-labeled nucleic-acid probe. After washing off the unbound and nonspecifically bound probe, hybridizing RNA molecules are revealed as autoradlographlc bands on a X-ray film 3,4

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References

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Author information

Authors and Affiliations

  1. Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore

    Paolo A. Sabelli

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  1. Paolo A. Sabelli
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Editor information

Editors and Affiliations

  1. University of Herfordshire, Hatfield, UK

    Ralph Rapley  & John M. Walker  & 

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© 1998 Humana Press Inc , Totowa, NJ

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Sabelli, P.A. (1998). Northern Blot Analysis. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_9

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  • DOI: https://doi.org/10.1007/978-1-59259-642-3_9

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-501-0

  • Online ISBN: 978-1-59259-642-3

  • eBook Packages: Springer Book Archive

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