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Monoclonal Antibodies

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Molecular Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Like all great discoveries, the procedure developed by George Kohler and Cesar Milstein for immortalizing antibody producing B-lymphocytes (1) is essentially simple, namely, mix B-lymphocytes from the spleen of an immunized rodent with a continuously proliferating B-lymphoma cell line, then induce their membranes to fuse to give a single antibody-secreting cell (hybridoma) that will proliferate indefinitely Importantly, each hybndoma will secrete antibodies with a specificity for only one antigenic epitope, I e., the antibodies are monoclonal It is this property, together with the ability to produce unlimited amounts of antibody, that has revolutionized the use of antibodies in many areas of biological and medical research, and has found important applications not only in clinical diagnosis and therapy, but also for large-scale purification of biological materials Indeed, monoclonal antibodies (MAbs) are essential tools for the cell and molecular biologist and their use has unlocked the doors behind which the function of many proteins and other cellular molecules would have lain hidden

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Authors and Affiliations

  1. Section of Immunology, Institute of Cancer Research, McElwatn Laboratories, Sutton, UK

    Christopher Dean & Helmout Modjtahedi

Authors
  1. Christopher Dean
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  2. Helmout Modjtahedi
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Editor information

Editors and Affiliations

  1. University of Herfordshire, Hatfield, UK

    Ralph Rapley  & John M. Walker  & 

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© 1998 Humana Press Inc , Totowa, NJ.

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Dean, C., Modjtahedi, H. (1998). Monoclonal Antibodies. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_44

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  • DOI: https://doi.org/10.1007/978-1-59259-642-3_44

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-501-0

  • Online ISBN: 978-1-59259-642-3

  • eBook Packages: Springer Book Archive

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